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Barfoed's test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu2O), which forms a brick-red precipitate. ::RCHO + 2Cu2+ + 2H2O → RCOOH + Cu2O↓ + 4H+ (Disaccharides may also react, but the reaction is much slower.) The aldehyde group of the monosaccharide which normally forms a cyclic hemiacetal is oxidized to the carboxylate. A number of other substances, including sodium chloride, may interfere. It was invented by Danish chemist Christen Thomsen Barfoed〔 and is primarily used in botany. The test is similar to the reaction of Fehling's solution to aldehydes. ==Composition== Barfoed's reagent consists of a 0.33 molar solution of neutral copper acetate in 1% acetic acid solution. The reagent does not keep well and it is therefore advisable to make it up when it is actually required.〔Bowen, Graham and Williams, ''A Students' Handbook of Organic Qualitative Analysis'', University of London Press, 1957, page 73〕 May store indefinitely according to several MSDS's.〔http://emc-msds.com/chemdocs/34/34634.pdf〕〔http://sargentwelch.com/pdf/msds/Barfoed_Reagent_77.00.pdf〕 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Barfoed's test」の詳細全文を読む スポンサード リンク
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